Identification of Virulence Genes of Helicobacter pylori by Random Insertion Mutagenesis
نویسندگان
چکیده
منابع مشابه
Identification of virulence genes of Helicobacter pylori by random insertion mutagenesis.
The complete genome of the gram-negative bacterial pathogen Helicobacter pylori, an important etiological agent of gastroduodenal disease in humans, has recently been published. This sequence revealed that the putative products of roughly one-third of the open reading frames (ORFs) have no significant homology to any known proteins. To be able to analyze the functions of all ORFs, we constructe...
متن کاملDetermination of Helicobacter pylori virulence-associated genes in duodenal ulcer and gastric biopsies
Background: Helicobacter pylori (H. pylori or Hp) has been strongly associated with the peptic ulcer diseases, chronic gastritis, ulcers, and gastric cancer. Genes associated with pathogenicity have been designated for H. pylori, and some of them appear to be related to more severe clinical consequences of the infection. The present study was conducted to determine cagA, vacA, cagE, iceA1, oipA...
متن کاملHelicobacter pylori virulence genes detected by string PCR in children
25 The accuracy of a nested PCR in gastric DNA obtained by string test for the diagnosis of 26 H. pylori infection in asymptomatic children was 94.0%. The cagA-positive toxigenic vacA 27 s1m1 strains were the most prevalent indicating that this population is early colonized by the 28 strains associated with gastric cancer. 29
متن کاملMicroevolution of Virulence-Related Genes in Helicobacter pylori Familial Infection
Helicobacter pylori, a bacterial pathogen that can infect human stomach causing gastritis, ulcers and cancer, is known to have a high degree of genome/epigenome diversity as the result of mutation and recombination. The bacteria often infect in childhood and persist for the life of the host. One of the reasons of the rapid evolution of H. pylori is that it changes its genome drastically for ada...
متن کاملVirulence of Helicobacter Pylori: Yesterday, Today and Tomorrow
Abstract Undoubtedly, H. pylori is the major human gastric pathogen, which infects the mucosal epithelium in 50% of world population. However, H. pylori infection is relatively prevalent globally the majority of infected individuals are asymptomatic. The recent epidemiological studies show that the various gastro intestinal complications can be the result of genetic variation in H. pylori stra...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Infection and Immunity
سال: 1999
ISSN: 0019-9567,1098-5522
DOI: 10.1128/iai.67.5.2433-2440.1999